Coding
PduD40-fMT

Part:BBa_K562018

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-18)


Salty_PduD40-fMT

This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to a metallothionein (fMT) from Fucus vesiculosus (from part BBa_K190019). The fMT gene product also carries a C-terminal HA epitope tag. Production of PduD40-fMT has been verified by Western immunoblotting (anti-mCherry). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-fMT in the Sargent Laboratory, Dundee, UK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 261
    Illegal BamHI site found at 473
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
    Illegal NgoMIV site found at 350
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None